Genomic Tools
Bioron GmbH offers you several "rare-cutters" - restriction endonucleases especially suitable for genomic research. All these enzymes passed through additional tests for successful and specific cleavage of high molecular weight DNA.
Sfi I
GGCCNNNN↓NGGCC
CCGGNNNNNCCGG
Smi
ATTT↓AAAT
TAAATTT↓A
Sbf I
CCTGCA↓GG
GGACGTCC
CciN I(iso- NotI)
GC↓GGCCGC
CGCCGGCG
A unique tool for generation of DNA fragments with long sticky ends
As an additional tool for genomic DNA fragmentation Bioron offers unique instrument - site-specific nickase N.Bst9. In contrast to the restriction enzymes, this enzyme cleaves only one DNA strand after the recognition sequence. Meanwhile, if two recognition sequences appear in close vicinity to each other, the enzyme acts exactly as a restriction endonuclease with 10 bp-long recognition sequence.
GAGTCNNNN↓NN..........NNNNNGACTC
CTCAGNNNNNNN.........NN↓NNNCTGAG
