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Vne I

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G↓TGCAC

CACGT↑­G

Purified from Vibrio nereis 18

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer O, 37oC

Recommended buffer:
O

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 20 units of the enzyme at 37oC.

Heat inactivation (20 min, 65oC):
Yes

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