Sma I
| Catalog # | Pack size | Price, Euro | SHOP |
| 246020 | 2000 u | 48,00 | Add to cart |
| 246100 | 10000 u | 192,00 | Add to cart |
Download: Datasheet Sma I
CCC↓GGG
GGG↑CCC
Purified from E.coli strain that carries Sma I gene from Serratia marcenscens
Concentration:
20000 units/ml
Activity assay:
Phage λ DNA/Hind III digest, SE buffer Y, 25oC.
Recommended buffer:
Y
Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut by T4 DNA ligase together with 10% of PEG.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of DNA with 40 units of the enzyme at 37oC.
Heat inactivation (20 min, 65oC):
Yes
