EcoR I
| Catalog # | Pack size | Price, Euro | SHOP |
| 222050 | 5000 u | 16,00 | Add to cart |
| 222250 | 25000 u | 64,00 | Add to cart |
Download: Datasheet EcoR I
G↓AATTC
CTTAA↑G
Purified from E.coli strain that carries the plasmid with EcoR I gene from Escherichia coli.
Concentration:
20-50000 units/ml
Activity assay:
Phage λ DNA, EcoR I buffer, 37oC
Recommended buffer:
EcoR I
Ligation/recutting assay:
After 40-fold overdigestion with EcoR I, more than 90% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 100 units of enzyme at 37oC.
Heat inactivation (20 min, 65oC):
Yes
