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BstDS I

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C↓CRYGG      

GGYRC­↑C

Purified from Bacillus stearothermophilus DS

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 65oC

Recommended buffer:
Y

Ligation/recutting assay: 
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and  recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 65oC.

Heat inactivation (20 min, 65oC):
No

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