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Acc65 I

Catalog #Pack sizePrice, EuroSHOP
2050101000 u32,00Add to cart
2050505000 u128,00Add to cart

Opens internal link in current windowDownload: Datasheet Acc65


G↓GTACC

CCATG↑­G 

Purified from Acinetobacter calcoaceticus

Concentration:
30-50000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37oC

Recommended buffer:    
W

Ligation/recutting assay:      
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 30 units of enzyme at 37oC.

Heat inactivation (20 min, 65oC):   
Yes

Note! Activity may be blocked by overlapping dcm methylation.

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