Nae I

Catalog #	Pack size	Price, Euro	SHOP
250122S	300 units	20,00	Add to cart
250122L	1.500 units	98,00	Add to cart

 

 

Cutting Sequence: GCC↓GGC

Isoschizomere: N/A

 

Substrate for unit definition: pBR322.

Reaction conditions: 10 mM Tris-HCl  (pH 7.9 at 25°C), 10 mM MgCl₂, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.

 

Storage buffer:  50 mM KCl,  10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol,   200 μg/ml BSA and 50% glycerol. Store at -20°C.

 

Absence of contaminants: Fifty units of NaeI do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C.  After ten-fold overdigestion with NaeI, greater than 80% of the DNA fragments can be ligated and recut with this enzyme.

 

Heat inactivation: 65°C for 20 minutes.

 

Note: Nae I exhibits site preferences.

 

Reaction Buffer Table