Bio Ni-IDA Mini Kit
| Catalog # | Preps | Price, Euro | SHOP |
| 810001 | 10 | 41.00 | Add to cart |
| 810005 | 50 | 199.00 | Add to cart |
This Product enable fast and convenient purification of recombinant polyhistidin-tagged proteins by immobilized metal ion affinity chromotography. Bio Ni-IDA is a dry silica-based resin precharged wih Ni2+ ions. Binding of protetein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. The chelating group of Ni-IDA is based on IDA(iminodiacetic acid), which enables strong and efficient binding of target protein onto the matrix. Due to a special manufacture proscess Bio Ni-IDA shows a low density of IDA ligand which eliminates non-specific interactions of contaminating prroteins with the adsorbent. As a result Bio Ni-IDA ensures Higher target protein purity.
Structure of IDA in complex with Ni2+
The Bio Ni-IDA Mini Kit contains:
- Bio Ni-IDA packed gravity-flow columns
- 1x Equilibration-Buffer
- 1x Washing-Buffer
- 1x Elution-Buffer
Specification of Bio Ni-IDA packed gravity-flow columns:
Protein capacity (6xHis-GFPuv,~32Da) | 800µg (40mg resign) |
| Application | Gravity flow columns |
| Physical form | Ready-to-use columns filled with dry matrix precharged with Ni2+ |
| Matrix | Macroporous silica |
| Density | 0,5 g/ml (1 g resign corresponds to 2ml bed volume) |
| Chelat group | IDA (iminodiacetic acid) |
| Mean particle size | 90µm |
| pH stability | 3-7,5 (<=2h) 2-3 or 7,5-8,5 (<=2h) |
| Storage | <=25°C |
| Recommended imidazole concentration for load/wash | 0 mM |
| Recommenden imidazole concentration for elution | <= 250 mM |
