• Home
• Hot-Start PCR
• PCR Products
• Thermocyclers
• Nucleotides
• DNA / Markers
• DNA-RNA Purification from AXYGEN
• Mutation analysis
• Other Enzymes
• Dialysis Membranes
• Plastic Consumables
Excellent Products from Bioron

• PCR Products
• Real time PCR
• Thermocyclers
• Dialysis Membranes
• DNA-RNA Purification
• Protein Purification
• Nucleotides
• DNA Marker, DNA, Plasmid
• Other Enzymes
• Mol-Bio Chemicals
• Restriction Enzymes
• Fluorescent detector
• Diagnostic Kits
• Mutation Analysis
  • Pharmacogen. mutation
  • Cancer related mutation
• Tubes, tips, Tissue culture
• DNA-RNA- Synthesizer
• Advertising 07/08
• Distribution

Download
Catalog 2007

GenePack DNA PCR tubes provide a simple and convenient analysis of Mutations assays for pharmacogenetics (Medicine incompatibilty test) study for CYP2D6, CYP2C9, CYP2C19, CYP1A1, CYP2E1, NAT2, DPD allelic variants detection.

All reagents are lyophilized and stabilized ...

..... for a convenient and simple usage, storage and transportation.

Benefits:

• improve the chance that the medicine will help

• lower the chance of servere side effects from medicine

• improve the patients safety.

Download: Manual of Pharmacogeneitc test kits

Applications in practical medicine

Each kit contains 100 tubes with lyophilised Master Mix with the following components:

• Taq DNA polymerase plus heat-sensitive Taq DNA inhibitors

• 4 primers for allele-specific PCR

• dNTP’s

• special salts and stabilizers

• loading dye (bromphenol blue)

• Separate Tube of dilution buffer

The Master-Mix can be transported stored at ambient temperature (+15-30 °C)  for more than 1 Year without any change in performance of mutation detection.
To start PCR one have to add genomic DNA, dilution buffer and water.  After PCR the product will be loaded on the Agarose gel (no need for loading dye).
The size of the fragments will indicate the presence or absence of the mutation in the sample studied. 

Introduction of Mutations assays for pharmacogenetics study

Analysis of allelic variants in genes coding for xenobiotic metabolizing enzymes was recognized as a valuable tool in determination of  possible toxic reaction of the patient to the particular medicine and in the individual selection of the correct drug for the optimal treatment. The most popular analysis has to do with the detection of the allelic variants for several CYP genes as well as for the detection of polymorphisms of genes coding for II-phase metabolizing enzymes.
The analysis is highly recommended before chemotherapy in cancer (DPD, CYP, NAT variants analysis), before anti-depression drugs selection (CYP, NAT variants analysis), before beta-blockers prescriptions for patients with cardiovascular diseases.

Benefit

• improve the chance that the medicine will help

• lower the chance of servere side effects from  the    medicine

• improve the patients safety.

• The genes tested today may be important for medicines that your patients will need in future

Figure 1.  

Principle of mutation detection

Each kit is designed for the detection of one specific mutation. The mutation detection is based on tetra primer allele-specific PCR. Flanking primers provide amplification of a region around possible mutation in all cases. Internal primers can discriminate mutation allele and wild-type allele by the corresponding amplicon size (Fig 1):

The Master-Mix can be transported and stored at ambient temperature at least for 1 Year without any change in performance of mutation detection.
To start PCR one have to add genomic DNA, dilution buffer and water.  After PCR the product will be loaded on the Agarose gel (no need for loading dye).
The size of the fragments will indicate the presence or absence of the mutation in the sample studied.

Copyright © BIORON GmbH