SNPase
SNPase was recognized as one of the best Polymerases in SNP detection by allele-specific PCR and micro sequencing.
| Catalog # | Pack size | Price, EUR | SHOP |
| 108205 | 500 units | 69,00 | Add to cart |
| 108225 | 2500 units | 276,00 | Add to cart |
| 108210 "HoT Start" | 500 units | 83,50 | Add to cart |
| 108250 "HoT Start" | 2500 units | 334,00 | Add to cart |
Download Datasheet: SNPase
Applications:
-SNP- genotyping , allele specific PCR, allele specific primer extension, mini
sequencing
-Highly specific PCR
-Multiplex PCR
-Highest fidelity PCR
-Real Time PCR with intercalating dyes (SYBR Green, Eva Green)
Description: SNPase is Taq DNA Polymerase with N-terminal deletion and prioprietary amino acids substitutions introduced into the active center of the enzyme. This modification causes dramatic increase of sensitivity of the enzyme to mismatches at 3’-end of the primer. Consequently , non-perfect annealing of the primers does not result in unspecific amplicons formation.
Features:
-High sensitivity to mismatches at 3’end of primers
-high fidelity of dNTPs and ddNTPs incorporation
-Lack of 5’- and 3’- exonuclease activity
-High specificity, low level of background PCR
Concentration: 20-25 µl
Storage conditions : -20°C
Supplied with : 5x Buffer without Mg 2+; MgCl2 solution ( 100mM);
Magnesium concentration: SNPase DNA polymerase provides successful PCR in range of Mg 2+ concentration from 2,5 mM to 4mM in the 1xreaction mixture. Increase of Mg 2+ concentration from 2,5 mMupto 4 mM can provide higher yield of PCR products . On the other hand low magnesium concentration favors specific PCR.
Associated activities: Endonuclease and exonuclease activities were not detected.
