DFS-Taq DNA Polymerase
| Catalog # | conc. | Pack size | Price, Euro | SHOP |
| 101005 | 5,0u/µl | 500 u | 69,00 quantity discount: see SHOP | Add to cart |
| 101025 | 5,0u/µl | 2500 u | 345,00 | Add to cart |
| 101100 | 5,0u/µl | 10000 u | 900,00 | Add to cart |
| 101500 | 5,0u/µl | 50000 u | 3000,00 | Add to cart |
Applications
- Standard PCR of DNA fragments up to 5 kb
- Realtime PCR
- Primer extension reaction
- PCR with bacterial DNA
Description
DFS (DNA Free Sensitive) Taq DNA Polymerase is a thermostable enzyme of approximately 94 kDa isolated from eubacterium Thermus aquaticus strain YT-1(1). This unmodified enzyme replicates DNA at 72°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5'--> 3' direction in the presence of magnesium ions and possesses a 5'--> 3' exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- or exonucleases. Taq DNA Polymerase leaves single 3'-dA nucleotide overhangs on their reaction products.
Features
- absolutely free from bacterial DNA
- with Bioron Buffer system for all purposes suitable
- high sensitivity: 6 molecules template may be enough
- efficient amplification of single copy genes
- high yields of products
Concentration: 5 units/µl
Storage conditions: -20oC
1 - without adding E.coli DNA (Test)
2 - with adding E.coli DNA (control)
Reference: 1. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644(Rus)
