DFS-Taq Blue
| Catalog # | conc. | Pack size | Price, Euro | SHOP |
| 101007 | 5,0u/µl | 500 u | 52,00 | Add to cart |
| 101012 | 5,0u/µl | 2500 u | 208,00 | |
| 101017 | 5,0u/µl | 10000 u | 582,50 | Add to cart |
| 101022 | 5,0u/ml | 50000 u | 2105,00 | Add to cart |
Download Datasheet:
Applications: DFS-Taq Blue is suitable for all regular applications – PCR, primer extension reactions etc. DFS-Taq Blue is free from bacterial DNA hence for the work with bacterial DNA. DFS-Taq Blue is recommended for reactions where visual control of reaction mixture preparation is desirable
Description: DFS-Taq Blue DNA Polymerase is a thermostable enzyme of approximately 94 kDa isolated from eubacterium Thermus aquaticus strain YT-1(1). This unmodified enzyme replicates DNA up to 5 kb length at 72°C and catalyzes the polymerization of nucleotides into duplex DNA in 5´-->3´ direction and 5´-->3´ exonuclease activity. The enzyme is highly purified and free of nonspecific endo- or exonucleases. Taq DNA polymerase leaves single 3'-dA nucleotide overhangs on their reaction products. The enzyme is stained in blue to provide visual control of enzyme handling and reaction mixtures preparation.
QC-Testing:
• storage (7 days at room temperature) test – no change in performance
• absence of exonuclease, endonuclease and nickase activities
• PCR with various templates – human and bovine genomic DNA, Phage Lambda DNA
• “no primers” test with Lambda DNA cycling without primers;
• “no template” test with the primers complementary to the conservative region of 16S
bacterial ribosomal genes
• Concentration 5000 units/ml
Sensitivity: of PCR reaction is high. As few as 6 DNA molecules were detected.
Concentration: 5000 units/ml
Unit definition: One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72°C.
Storage buffer: 10 mM K-phosphate, pH 7.4, 0.1 mM EDTA, 50% glycerol, 0.1% Triton X-100, 0.1% Tween 20.
Reaction buffer (x10)” incomplete”: 160 mM (NH4)2SO4, 670 mM TrisHCl pH 8.8, 0.1% Tween-20
Reaction buffer (x10) “complete”: 160 mM (NH4)2SO4, 670 mM TrisHCl pH 8.8, 0.1% Tween-20, 25 mM MgCl2
Reaction buffer (x10) “complete II KCl”: 500 mM KCl, 100 mM TrisHCl pH 8.8, 0.1% Tween-20, 15 mM MgCl2
Additionally provided: 1 Tube MgCl2 (100 mM); Recommended MgCl2 concentration 1.5 mM – 6 mM
Storage condition temperature is -20°C
