DFS-Master Mix Blue (2x)
| Catalog # | Pack size | Price, Euro | SHOP |
| 101705 | 100 rcs (2x1,25ml) | 55,00 | Add to cart |
| 101725 | 5 x 100 rcs (10x1,25ml) | 245,00 | Add to cart |
PCR-Master Mix information : The Master Mixes are calculated for 50 µl per reaction. Your advantage: if you work with 25 µl reaction volume you can run 200 tests.
Download: Datasheet DFS-Master Mix Blue (2x)
Applications:
- Standard PCR up to 5 kb products
- PCR with bacterials templates
- Amplification of difficult c-DNA and genomic targets
- single copy PCR
- multiplex amplification
Features:
- visible control of PCR set-up
- ease of use, reduction of pipetten errors
Description 2x DFS-Master Mix Blue is an optimized ready-to-use PCR mixture of DFS-Taq DNA Polymerase, PCR buffer, MgCl2 and dNTPs. The mixture contains bromphenole blue to provide visual control of the reaction mixture preparation. 2x PCR Master Mix contains all components for PCR, except DNA template and primers. The mixture contains DFS-Taq DNA polymerase – Taq DNA polymerase without traces of E. coli DNA, but with the increased ability to detect low-copy number genes (Bioron GmbH, cat# 
101005,). Due to the usage of DFS-Taq DNA polymerase the mixture can be recommended not only for all regular applications, but also for templates which contains E. coli DNA sequences.
2x Master Mix Blue Composition
• Taq DNA Polymerase (recombinant) in reaction buffer: 0.1 units/µl
• 32 mM (NH4)2SO4
• 130mM TrisHCl, pH 8.8 at 25°C
• 0,02% Tween-20
• Bromphenole Blue 0,001%
• 5,5mM MgCl2
• dNTPs (dATP, dCTP, dGTP, dTTP): 0.4mM of each
Performance and purity tests Tested for the absence of endodeoxyribonucleases and exodeoxyribonucleases. Performance is tested on single-copy genes of human and mouse DNA.
Endodeoxyribonuclease Assay No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 25µl of 2x PCR Master Mix with 1µg of pUC19 DNA in 50µl for 4 hours neither at 37°C nor at 70oC
Storage MasterMix should be stored at -20oC. Repeated (up to 10 times) freezing – defreezing do not cause the reduction in PCR performance, storage at +4 oC for 1 week do not cause the reduction in PCR performance.
Protocol: It’s strongly recommended to settle-up all reactions in ice to avoid formation of unspecific products or primer-dimers formation.
| 50 µl reaction volume | 50 µl reaction volume |
| component | volume | final conc. | volume | final conc. |
| 2x PCR Master mix | 25 µl | 1x | 12.5 µl | 1x |
| MgCl2 | variable | variable | variable | variable |
| forward primer | variable | 0.1 - 1 µl | variable | 0.1 - 1 µl |
| reverase primer | variable | 0.1 - 1 µl | variable | 0.1 - 1 µl |
| Template DNA | variable | 10 pg-1µg | variable | 10 pg-1µg |
| Sterile Deionized water | up to 50 µl | - | up to 25 µl | - |
• Gently vortex the sample and briefly centrifuge to collect all drops to the bottom of the tube.
• Overlay the sample with mineral oil or add an appropriate amount of wax if the thermal cycler is not equipped with a heated lid.
• Place the samples in a thermocycler and start the optimal PCR program.
If MgCl2 is not added to the reaction mixture, final concentration of MgCl2 in the reaction mixture will be 2,75mM. Addition of 1 µl of 100 mM MgCl2 into the mixture results in concentration of MgCl2 4,75mM in the reaction mixture for 50 µl reaction volume.
