Tth DNA Polymerase
| Catalog # | Pack size | Price, Euro | SHOP |
| 104005 | 500 u | 80,00 | Add to cart |
| 104025 | 2500 u | 320,00 | Add to cart |
Datasheet: Tth DNA Polymerase
Description:
Tth DNA Polymerase is a thermostable enzyme of approximately 94 kDa isolated from eubacterium <em>Thermus thermophilus</em> strain HB8. This enzyme replicates DNA at 74°C (please see protocol in datasheet). It reveals RNA-dependent DNA-polymerase activity in the presence of Mn2+ ions. The concentration of RNA template for effective reverse transcription with Tth DNA polymerase should be higher if to compare with reverse transcription directed by Reverse Transcriptases (M-MuLV, AMV).
Concentration:
5000units/ml
Storage buffer:
10 TrisHCl, pH 7.5, 300 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol.
Unit definition:
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 70oC.
Supplied Reaction buffers:
1. RT-PCR buffer, one-step reaction (5 x): 250 mM bicine/KOH, pH 8.2 (25°C); 575 mM K-acetate, 40% glycerol (v/v)
2. 10 x Reverse transcription buffer: 100 mM Tris-HCl (pH 8.9 at 25°C), 900 mM KCl
3. 10 x Tth PCR buffer: 100 mM Tris-HCl (pH 8.9 at 25°C), 1M KCl, 500 µg/ml BSA, 0.5% Tween 20 and 15 mM MgCl2
4. Mn2+: 1 - 2 mM for RNA - dependent c-DNA synthesis. (supplied as MnCl2 in a separate tube).
5. Mg2+: 3-6 mM for PCR reaction (supplied as MgCl2 in a separate tube).
Recommended concentration of MgCl2: 3 - 6 mM
Storage conditions: -20oC
