Taq T
| Catalog # | Pack size | Price, Euro | SHOP |
| 117005 | 500 u | 120,00 | Add to cart |
| 117025 | 2500 u | 500,00 | Add to cart |
Download: Datasheet (PDF)Description:
Taq DNA Polymerase, modified (Taq-T) - is a modified form of the enzyme from the thermophilic eubacterium Thermus aquaticus. This enzyme has a modification in dNTP binding site. Due to the modification enzyme incorporates dUTP and dideoxynucleotides more efficiently in comparison with Taq DNA Polymerase. Enzyme may be used for cycle sequencing, recommended for dUTP/ bio-dUTP incorporation reactions.
Storage and dilution buffer:
10 mM K-phosphate bufffer, pH 7.4, 100 mM KCl, 0.1 mM EDTA, 50% glycerol, 0.1% Triton X-100, 0.1% Tween-20.
Unit definition:
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 72oC.
Reaction buffer (x10) incomplete:
160 mM (NH4)2SO4, 670 mM TrisHCl pH 8.8, 0,1% Tween-20, plus one tube plus one Tube MgCl2 (100 mM)
Reaction buffer (x10) complete:
160 mM (NH4)2SO4, 670 mM TrisHCl pH 8.8, 0,1% Tween-20, 25 mM MgCl2
Reaction buffer (x10) complete II KCl:
500 mM KCl, 100 mM TrisHCl pH 8.8, 0,1% Tween-20, 15 mM MgCl2
Recommended MgCl2 concentration :
1.5 mM - 6 mM
Storage :
-20°C
Concentration :
5 units/ml
