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RNase Inhibitor

Ribonuclease Inhibitor (RNase Inhibitor)

Catalog #Pack sizePrice, EuroSHOP
1053102000 units54,00Add to cart
10535010000 units199,00Add to cart

 

Datasheet: RNase Inhibitor

Description:
Ribonuclease Inhibitor has broad spectrum RNase inhibitory properties. The 50 kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. The product is purified using a combination of ion exchange and affinity chromatography.

Features:
Inhibits Common Eukaryotic RNases: Ribonuclease Inhibitor has broad-spectrum RNase inhibitory properties, including RNase A, RNase B, RNase C and human placental RNase. Does not inhibit RNase T1, S1 nuclease, RNase from <em>Aspergillus</em>, RNase H, RNase ONETM Ribonuclease.

Compatibility:
Ribonuclease Inhibitor does not inhibit SP6, T7 or T3 RNA Polymerase; AMV or M-MLV Reverse Transcriptase; or Taq DNA Polymerase.

Broad pH Range:
Active over a broad pH range (pH 5-8).

Applications: 

  • RT-PCR Useful in any applications where eukaryotic RNase contamination is a potential problem.
  • Protection of mRNA in cDNA synthesis reactions.
  • In vitro transcription/translation.
  • Improvement of in vitro virus replication. I
  • mprovement of RNA translation in homologous systems.
  • Preparation of RNase-free antibody.

Storage Conditions: Store at -20°C.

Storage Buffer:
20 mM HEPES-KOH (pH 7.6 at 4°C), 50 mM KCl, 8 mM DTT and 50% (v/v) glycerol.

Unit Definition:
One unit is defined as the amount of Ribonuclease Inhibitor required to inhibit the activity of 5 ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.

Concentration: 
20 u/µl

Quality Control Tests: 
SDS-PAGE - purity, DNase, RNase, endonuclease/nickase.

Source: 
Human Placenta

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