Red SuperHoTTaq (Hot-start) DNA Polymerase
| Catalog # | Pack size | Price, Euro | SHOP |
| 119005 | 200 u | 80,00 | Add to cart |
Datasheet Red SuperHoTTaq (Hot start) polymerase
Unit Definition: One unit defined as the amount of the enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72oC.
Recommended Reaction Buffer (x1): 16mM (NH4)2SO4, 67 mM Tris-HCl (pH 8.8); 1,5-7mM MgCl2 0.01% Tween-20
Reaction buffer (x10) "incomplete" supplied: 160 mM (NH4)2SO4, 670mM TrisHCl pH8.8, 0.1% Tween-20
Reaction buffer (x10) "complete" supplied: 160 mM (NH4)2SO4, 670mM TrisHCl pH8.8, 0.1% Tween-20, 25mM MgCl2
Reaction buffer (x10) "complete II KCl" supplied: 500 mM KCl, 100 TrisHCl pH8.8, 0.1% Tween-20, 15mM MgCl2
Storage Buffer: 10mM Tris-HCl (pH 7.0); 50mM KCl; 0.1mM EDTA; 50% glycerol
Storage conditions: Long term storrage: -20oC
Concentration: 5000 units/ml
Recommended PCR conditions: Use PCR conditions optimized for Taq DNA Polymerase. In the case of low amount of DNA template, additional cycles may be used.
Applications: Complex genomic or cDNA templates, low copy numbers targets, large numbers of thermal cycles, multiplex PCR
