1 Tube RT-PCR Kit "Hot-Start" for qPCR
| Catalog # | Pack size | Price, Euro | SHOP |
| 122305 | 50 rcs | 197,50 | Add to cart |
| 122325 | 500 rcs | 1485,00 | Add to cart |
Datasheet: One Tube RT-PCR Kit "Hot-Start" for qPCR and Protocol
The RT-PCR kit is designed especially for simple and effective one-tube RT-PCR. The reverse transcription step is directed by M-MuLV reverse transcriptase, as soon as cDNA is synthesized, a chemically modified "Hot-Start" Taq DNA polymerase, is activated by heating up the reaction 10 min at 95oC. As soon as the Taq-star enzyme is activated, regular PCR procedure is performed. An optimized reaction buffer provides good performance both for Reverse Transcriptase and for Taq DNA Polymerase. The buffer qA provides good performance in TaqMan assay and in SybrGreen Real Time analysis as well.
The kit for one tube RT-PCR contains:
1. Reaction buffer qA (5x) MgCl2
15 mM )
2. dNTPs 5 mM
3. Mixture of the enzymes
4. DNAse-RNAse free water
Storage:
at -20°C
