Nucleotides
set of dNTP's (100mM)Spectral characteristics of dNTPs
| dNTP | Spectra-Acid | Spectra-Neutral | Spectra-Alkaline | ||||||
| pH | lammax | am/10-3 | pH | lammax | am/10-3 | pH | lammax | am/10-3 | |
| dATP | 2.0 | 258 | 14.8 | 7.0 | 259 | 15.4 | 12.0 | 260 | 15.4 |
| dCTP | 2.0 | 280 | 13.1 | 7.0 | 272 | 9.1 | 12.0 | 273 | 8.6 |
| dGTP | 2.0 | 255 | 12.3 | 7.0 | 252 | 13.7 | 12.0 | 262 | 11.7 |
| dTTP | 2.0 | 267 | 9.6 | 7.0 | 267 | 9.6 | 12.0 | 267 | 7.8 |
| dUTP | 2.0 | 262 | 10.0 | 7.0 | 262 | 10.0 | 12.0 | 261 | 8.0 |
Deoxynucleoside 5'-triphosphates are chemically synthesized from deoxynucleoside 5'-monophosphate and purified by Ion-Exchange and reverse phase chromatography (purity > 98%).
dNTPs effectively direct PCR with the amplicon size up to 10 kb. Each particular dNTP is free from any traces of other dNTPs. dNTPs are supplied in sodium salts, in solutions and in powder. Applications of dNTPs include PCR, long PCR, Real Time PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and labeling.
Recently a very high sensitivity of Pfu DNA polymerase to traces of pyrophosphate has been discovered. It is quite difficulte to find traces of pyrophosphates in dNTPs, so dNTPs from some commercial sources can cause problems in PCR with Pfu DNA Polymerase.
All dNTP batches from Bioron are carefully tested for effective PCR both with Taq DNA Polymerase and Pfu DNA Polymerase. Moreover, the products are tested for the absence of other inorganic salts.
dNTPs provided by Bioron are RNase free - a very sensitive fluorescent test for RNase activity provided by Ambion can not detect traces of RNase in the preparations.
Modified dNTPs offered by Bioron are convenient tools for DNA labeling. dATP, dCTP, dUTP are provided with amino-linkers to facilitate the introduction of any suitable label into DNA. Fluorescent-labelled dUTPs can be used for simple DNA labelling by PCR or nick-translation. Several popular chain-terminators as d4T-TP and AZT-TP can be used in analysis of viral Reverse Transcriptase features (e.g. HIV sensitivity to AZT or d4T).
