GenePack DNA/RNA PCR tests systems provide simple and convenient analysis of infection agents presence in biological samples based on PCR techigue.
All reagents are lyophilized and stabilized ..... for R&D only
All reagents required for PCR (Taq DNA polymerase, dNTPs, specific primers, salts and stabilizers) or for Reverase transcription (Enzyme, placental Ribubuclease inhibitor) are lyophilized in PCR tubes (0,2 ml).
Only the sample to be analyzed and specific buffers (provided in the kit) should be added before thermocycling.
All reagents are lyophilized and stabilized, so GenePack DNA PCR tests can be transported at ambient temperature (+15-30 °C) and stored at +2-8°C for a long time (more then 1 Year).
Please note: The test systems are not CE-marked
Test system for detection specific DNA | Test system for viral RNA detection |
 Complete list of all DNA test systems |
Download Datasheet/manual for: DRY-Mix Gene Pack for infection diseases (DNA) download Datasheet/manual for: DRY-Mix Gene Pack for infection diseases (RNA)
Introduction:
DNA/RNA purification kits from any supplier can be used for DNA purification from bio-samples. We recommend the OTP-reagent from Bioron for the fastes method to purify DNA for PCR. A maximal volume of 10 microliters of DNA purified from biosamples should be added into GenePack DNA/RNA test tube, mixed with 10 microliters of dilution buffer. If necessary add water (PCR grade, sterile) to a final volume of 20 µl and the mixture is ready for thermocycling.
Analysis:
After thermocycling the sample should be loaded on the agarose gel, the gel is to be stained by Ethidium Bromide after electrophoresis, positive control samples should reveal the specific band, no band should appear in negative control samples. The presence or absence of the specific band indicate the presence or absence of the specific DNA in the biosample tested.
Sensitivity:
The sensitivity of the test is 10-100 copies of DNA template in 10 microliters of the sample depending of the test. The problem of PCR inhibition and DNA losses during sample preparation can be solved by the addition of “Universal control DNA” into the sample just before DNA purification step.
Test system for detection specific DNA | Test system for viral RNA detection |
| Complete list of all DNA test systems |
