Special Application

SD Polymerase

SD Polymerase is a novel artificial thermostable polymerase providing a strong strand displacement activity. Unlike natural enzymes with strong strand displacement activity as Phi29 or Bst Polymerase, which are active below 68 °C only, SD Polymerase is stable up to 93 °C. This allows running isothermal amplification like LAMP with initial heating-up for increased specificity.
SD Polymerase has 5’ – 3’ polymerase and 5’ – 3’ strand displacement activities. It does not have any exonuclease activity. The enzyme does 3’-A-overhangs. SD Polymerase is also available as a HotStart version.

Product Cat.No. Packsize Quantity  
SD Polymerase, 10 U / µl
[PDS]
108702 200 U Add to cart
SD Polymerase, 10 U / µl
108710 1000 U Add to cart
SD Polymerase, 50 U / µl
[PDS]
108800 1000 U Add to cart
SD Polymerase, 50 U / µl
108850 5000 U Add to cart
SD Polymerase Hotstart, 10 U / µl
[PDS]
108902 200 U Add to cart
SD Polymerase Hotstart, 10 U / µl
108910 1000 U Add to cart

SD Polymerase is used in our WGA-Kit for whole genome amplifcation:  One Step WGA Kit

Features

  • Available in high and low concentrations and as a HotStart version
  • Higher yields, speed and efficiency compared to Taq Polymerase
  • Suitable for amplification of short (100 bp) and long (20 – 30 kb) DNA fragments
  • Suitable for simple (e.g. plasmids) and complex (e.g. genomic DNA) templates

Applications

  • Suitable for different PCR methods, also long-range PCR
  • PCDR (Polymerase Chain Displacement Reaction)
  • LAMP (Loop-mediated isothermal amplification)

Publications

BioRxiv (WGA Kit)

GenomeWeb 08/2014

Biotechniques 08/2014