Special Application

One Step WGA Kit

BIORON´S One Step WGA Kit, developed and produced at BIORON GmbH, is an improved version of the DOP (degenerated oligo PCR) with optimized primer sequences and BIORON’s unique SD Polymerase. This enzyme combines the strand displacement capability with heat stability up to 92 °C. Due to these features it is possible to use this enzyme for both amplification steps without additional pipetting or hands on time. The Kit contains everything that is necessary for amplification of human genomic DNA from single cells or low copy templates. Each Kit contains a 2.5x MM (Master Mix without enzyme); 10 U/µl SD Polymerase; human genomic DNA as positive control and PCR grade water.

Product Cat.No. Packsize Quantity  
One Step WGA Kit

[PDS]
108725 25 rcs Add to cart
One Step WGA Kit

108750 50 rcs Add to cart

see also: SD Polymerase

Features

  • Amplification finished in less than 2 hours
  • Different genomic amplification coverage than other enzymes
  • Long fragments up to 3 Kb
  • One Enzyme for the whole genome amplification

Applications

  • Single cell DNA analysis
  • Next Generation Sequencing (NGS)
  • SNP Genotyping
  • qPCR- and PCR-based mutation detection
  • STR and RFLP analysis
  • Array technologies (comparative genomic hybridization)

References

BioRxiv

WGA Kit.03-2017.jpg