BIORON GmbH
 

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SSB-HotTaq DNA Polymerase

SSB-HotTaq is highly suited for hot-start PCR, qPCR and regular PCR applications. SSB-HotTaq minimizes primer/dimer formation, reduces unspecific oligonucleotide priming and, therefore, enhances target-specific amplification. SSB-HotTaq, which possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity is a mixture of the thermostable Taq DNA Polymerase with a single-strand DNA binding (SSB) protein. The SSB protein binds to the PCR primers, preventing unspecific priming and amplification activity at lower temperatures. This allows for a convenient setup of PCR reactions at room-temperature, which also facilitates binding of the SSB protein to the oligonucleotides. The SSB protein becomes denatured during the initial denaturation step when amplification reactions are heated to 94 – 95 °C and bound oligonucleotides are now released for specific target priming. This allows for hot-start PCR in which the DNA polymerase finds an initiation complex for extension only after the first primer annealing step. SSB-HotTaq can be used in typical Taq-based cycling protocols.

 

Product name Cat.No. Packsize  
SSB-HotTaq DNA Polymerase, 5 u/ µl

[PDS] 		119702 200 U Add to cart
SSB-HotTaq DNA Polymerase, 5 u/ µl

[PDS] 		119710 1000 U Add to cart

Concentration

5 units/µl

Unit Definitions

One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72 °C.

Features

  • significant minimized formation of primer/dimer,
  • reduction of unspecific oligonucleotide priming
  • enhances target-specific amplification
  • no change or optimization of protocol necessary

 

Application

  • qPCR
  • Hot Start PCR
  • Regular PCR

Recommended PCR Conditions

Use PCR conditions optimized for Taq DNA Polymerase. In the case of a low amount of DNA template, additional cycles should be used.

Supplied Reaction Buffers

Complete NH 4 Reaction buffer Ammonium buffer with 25 mM MgCl 2
Incomplete NH 4 Reaction buffer Ammonium buffer without MgCl 2
Complete KCl Reaction buffer Potassium buffer 15 mM MgCl 2
Included MgCl 2 (100 mM)

We recommend Ammonium buffer for increased yield of PCR products and potassium buffer for increased specificity of PCR. MgCl 2 concentration should be optimized for each particular primer-template combination, meanwhile PCR is effective with 2.5 mM of MgCl 2 in the majority of cases.

Storage

-20 °C

 

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