E. coli Uracil-DNA Glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or less bases).

Concentration

10-20 u/?l

Storage buffer

• 20 mM Tris-HCl
• 50 mM NaCl, pH 8.0
• 0.1 mM EDTA
• 50% glycerol

Unit definition

One unit of activity is the amount of enzymes required to release 60 pmol of uracil per minute from double-stranded uracil-containing DNA.

Reaction buffer (10x)

• 200 mM TrisHCl, pH 8.0
• 10 mM EDTA
• 10 mM DTT

The enzyme is active in water or TE buffer

Storage conditions

-20°C