Lambda DNA (cI857 Sam 7 )/Hind III Markers are prepared by digesting Lambda DNA with Hind III, followed by heat-inactivation of the enzyme. Afterwards the DNA fragments are ethanol-precipitated and resuspended in storage buffer.

Fragment sizes (Base Pairs)

23,130; 9,416; 6,557; 4,361; 2,322; 2,027; 564, 125

Storage buffer

• 10 mM Tris-HCl (pH 8.0)
• 1 mM EDTA

Concentration

0.2-0.5 µg/µl

Number of Bands

8

Storage conditions

-20°C

Attention!

The cohesive ends of fragments 1 and 4 may cause formation of extra band 27491 bp. The fragments can be separated by heating to 65°C for 3 minutes before loading the sample on the gel.