50 bp plus DNA Ladder is ideal for determining the size of double-stranded DNA from 50 to 700 base pairs. The ladder consists of ten DNA fragments. Five fragments are the products of the restrictions of special plasmid by RsaI restrictase. The 350, 250,150 and 100 bp fragments are PCR products. Pfu DNA polymerase was used for amplification. The 50 bp fragment is synthetic. The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are blunt-ended.

Fragment sizes (Base Pairs)

700, 500, 400, 350, 2×300, 250, 200, 150, 100, 50

Concentration

0.2 mg/ml

Storage buffer

The ladder is available in 2 buffers:

1. No stain – 10mM Tris-HCl, pH 7.5, 1 mM EDTA,10mM NaCl
2. Blue – 10mM Tris-HCl, pH 7.5, 1 mM EDTA,10mM NaCl, 6% glycerol, 0.05% Xylene Cyanol

Storage conditions

-20°C

Loading

The recommended amount of size marker to load on agarose gel is 0.5 μg/lane.