100 bp RAINBOW extended DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 2 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 – 1,500 base pairs. The 500 bp fragment and the 1,000 bp fragment are present at increased intensity to allow easy identification. All fragments are blunt-ended.

Fragment sizes (Base Pairs)

100 (20 ng); 200 (20 ng); 300 (30 ng); 400 (50 ng); 500×2 (150 ng); 600 (80 ng); 700 (70 ng); 800 (110 ng); 900 (120 ng); 1000×2 (200 ng); 1500 (150 ng).

Concentration

0.1 µg/µl

Storage buffer

• 10 mM Tris-HCl (pH 8.0)
• 5 mM NaCl
• 1 mM EDTA
• 10% glycerol
• 0.009% xylene cyanol FF
• 0,08% orange G

Storage conditions

-20°C

Usage recommendation

The 100 bp RAINBOW eXtended DNA Ladder was not designed for precise quantification of DNA mass, but can be used for approximating the mass of DNA in comparably intense samples of similar size.
We recommend loading of 1 µg of the DNA Ladder per line.