Vha464 I

C↓TTAAG

GAATT↑C

Purified from Vibrio harveyi 464

Concentration:
20000 units/ml

Activity assay:
Phage λ DNA, SE buffer G, 37^oC

Recommended buffer:
G

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 40% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 37^oC.

Heat inactivation (20 min, 65^oC):
Yes

Copyright © BIORON GmbH