Sph I
| Catalog # | Pack size | Price, Euro | SHOP |
| 248002 | 200 u | 40,00 | Add to cart |
| 248010 | 1000 u | 160,00 | Add to cart |
Download: Datasheet Sph I
GCATG↓C
C↑GTACG
Purified from Sphaerotilus species
Concentration:
10000 units/ml
Activity assay:
Phage λ DNA, SE buffer G, 37oC
Recommended buffer:
G
Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of DNA with 10 units of the enzyme at 37oC.
Heat inactivation (20 min, 65oC):
Yes
