endecnplse

Sfr303 I

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2810101000 u24,00Add to cart
2810505000 u96,00Add to cart

Opens internal link in current windowDownload: Datasheet Sfr303 I (Sac II)


CCGC↓GG

GG­↑CGCC

Purified from Streptomyces fradiae 303

Isoschizomer Sac II

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37oC.

Recommended buffer:
B

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37oC.

Heat inactivation (20 min, 65oC)  :
Yes

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