Mlu I

A↓CGCGT

TGCGC↑A

Purified from Micrococcus luteus

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer O, 37^oC

Recommended buffer:
O

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 37^oC.

Heat inactivation (20 min, 65 ^oC) :
Yes

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