Hind III

A↓AGCTT

TTCGA↑A

Purified from Haemophilus influenzae Rd

Concentration:
20-50000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37^oC

Recommended buffer:
W

Ligation/recutting assay:
After 30-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 60 units of enzyme at 37^oC.

Heat inactivation (20 min, 65^oC) :
No

Copyright © BIORON GmbH