endecnplse

Bsc4 I

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268005500 u48,00Add to cart
2680252500 u192,00Add to cart

Opens internal link in current windowDownload: Datasheet Bsc4 (Bsl I)


CCNNNNN↓NNGG

GGNN↑­NNNNNCC

Purified from Bacillus schlegelii 4

Isoschizomer Bsl I

Concentration:
20-60000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 55oC.

Recommended buffer:
W

Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16h incubation of 1 mg of λ DNA with 60 units of enzyme at 55oC.

Heat inactivation (20 min, 65oC)  :
No

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