endecnplse

Bgl I

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GCCNNNN↓NGGC

CGGN↑­NNNNCCG

Purified from Bacillus globigii

Concentration:  5-30000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37oC

Recommended buffer:
W

Ligation/recutting assay:    
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 37oC.

Heat inactivation (20 min, 65oC):
Yes

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