Sla I (Xho I)

Catalog #	Pack size	Price, Euro	SHOP
250134S	5.000 units	20,00	Add to cart
250134L	25.000 units	98,00	Add to cart

 

 

Cutting sequence: C↓TCGAG

Isoschizomere: Xho I

 

Buffer supplied: 10x SH.

 

Substrate for unit definition: λ DNA (Hind III digest).

 

Reaction conditions: 150 mM NaCl, 10 mM Tris-HCl  (pH 7.9 at 25oC), 10 mM MgCl₂, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.

 

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.

 

Absence of contaminants: Fifty units of SlaI do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA (HindIII digest) at 37°C.  After ten-fold overdigestion with Sla I, more than 98% of the  DNA fragments can be ligated and recut with this enzyme.

 

Heat inactivation: 65°C for 20 minutes.

 

Reaction Buffer Table