Sla I (Xho I)
| Catalog # | Pack size | Price, Euro | SHOP |
| 250134S | 5.000 units | 20,00 | Add to cart |
| 250134L | 25.000 units | 95,00 | Add to cart |
Cutting sequence: C↓TCGAG
Isoschizomere: Xho I
Buffer supplied: 10x SH.
Substrate for unit definition: λ DNA (Hind III digest).
Reaction conditions: 150 mM NaCl, 10 mM Tris-HCl (pH 7.9 at 25oC), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Absence of contaminants: Fifty units of SlaI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA (HindIII digest) at 37°C. After ten-fold overdigestion with Sla I, more than 98% of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes.
Reaction Buffer Table
