Nco I

Catalog #	Pack size	Price, Euro	SHOP
250123S	600 units	20,00	Add to cart
250123L	3.000 units	98,00	Add to cart

 

 

Cutting Sequence: C↓CATGG

Isoschizomere: N/A

 

Source: Nocardia corallina.

 

Buffer supplied: 10x Nco I.

 

Substrate for unit definition:  λ DNA.

 

Reaction conditions: 100 mM NaCl,  50 mM Tris-HCl  (pH 7.9 at 25°C),  10 mM MgCl₂, 1 mM dithiothreitol, 0.02% Triton X-100, 100 μg/ml BSA.  Incubate at 37°C.

 

Storage buffer:  50 mM KCl,  10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.

 

Absence of contaminants:  Fifty units of NcoI do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After ten-fold overdigestion with NcoI, more than 95% of the DNA fragments can be ligated and recut with this enzyme.

 

Heat inactivation: 65°C for 20 minutes.

 

Star activity: Conditions of high enzyme concentration or glycerol concentration > 5%, may result in star activity.

 

Reaction Buffer Table