Nco I
| Catalog # | Pack size | Price, Euro | SHOP |
| 250123S | 600 units | 20,00 | Add to cart |
| 250123L | 3.000 units | 95,00 | Add to cart |
Cutting Sequence: C↓CATGG
Isoschizomere: N/A
Source: Nocardia corallina.
Buffer supplied: 10x Nco I.
Substrate for unit definition: λ DNA.
Reaction conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 0.02% Triton X-100, 100 μg/ml BSA. Incubate at 37°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Absence of contaminants: Fifty units of NcoI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After ten-fold overdigestion with NcoI, more than 95% of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes.
Star activity: Conditions of high enzyme concentration or glycerol concentration > 5%, may result in star activity.
Reaction Buffer Table
