BseB I
| Catalog # | Pack size | Price, Euro | SHOP |
| 250108S | 4.500 units | 30,00 | Add to cart |
| 250108L | 22.500 units | 155,00 | Add to cart |
Cutting sequence: CC↓(A/T)GG
Isoschizomere: BstN I
Source: Bacillus staerothermophilus.
Buffer supplied: 10x M.
Substrate for unit definition: λ DNA.
Assay conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 60°C under parafin oil.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Absence of contaminants: Fifty units of BseBI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 60°C. After ten-fold overdigestion with BseBI, less than 50% of the DNA fragments can be ligated.
Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.
Heat inactivation: No.
Note: BseBI-cut DNA is difficult to ligate with T4 DNA Ligase. Ligation is enhanced in the presence of 15% PEG4000.
Reaction Buffer Table
