endecnplse

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The compact line of Bioron offers the core restriction endonucleases you need for your daily work. All enzymes are produced at an ISO 9001 certificated company under high-tech conditions. The concentration of the restriction endonucleases is adjusted to 8-12 units per microliter.

Alu I - BseC I BshF I - Hpa IKpn I - Nru IPst I - Sla ISma I - Xba I
Alu IBshF IKpn IPst ISma I          
ApaL IBsiS I Mbo IPvu IISnaB I
Asu II BssA I MspC I Rsa ISph I
BamH IBstE IINae ISal ISseB I
Bcl ICspA I Nco ISau3A I

Bgl I

EcoR INhe ISca ISst I
Bgl IIEcoR VNot ISfi ISty I
BseA I Hind IIINru ISgrB I (Sac II)Taq I
BseB I Hinf ISla IXba I
BseC IHpa I


Isoschizomeres to Bioron's endonucleases:

Isoschizo-mereBioron's
enzyme		    Isoschizo-mereBioron's
enzyme		   Isoschizo-mereBioron's
enzyme		   Isoschizo-mereBioron's
enzyme
Aat ISseB IBsiX IBseC ICbi IAsu IINgoM IV Nae I
Acc IIIBseA IBsp106 IBseC ICfr42 ISgrB INspVAsu II
Afl IIMspC IBsp119 IAsu IICla IBseC IPaeR7 ISla I
Age ICspA IBsp13 I BseA ICsp45 IAsu IIPal IBshF I
AsiA ICspA IBspD IBseC IEco147 ISseB IPinA I CspA I
Ban III BseC IBspEI BseA IEcoR IIBseB IPm55 II SseB I
Bfr IMspC IBspT IMspC IHae IIIBshF IPspG I BseB I
Bpu14 IAsu IIBspX IBseC IKpn IBseA ISac IISgrB I
Bsa29 IBseC IBst2U I BseB IKsp ISgrB ISfr274 ISla I
Bsc IBseC IBst98 IMspC ILsp IAsu IISfr303 I SgrB I
Bsh IBshF IBstB IAsu IIMro IBseA ISfu IAsu II
BshT ICspA IBstN IBseB IMroN INae ISst II SgrB I
BsiC IAsu IIBstO I BseB IMsp IBsiS IStu ISseB I
BsiL IBseB IBsu15 IBseC IMva IBseB IThlSla I
BsiM IBseA IBsuR IBshF INgoA IVNae IXho I SseB I

 

 


Quality Test Methods

Overnight Assay for Nuclease Contamination
BIORON uses an overnight assay as a qualitative determination of enzyme purity and of a lack of nonspecific DNases. In this assay, increasing amounts of the enzyme are added to a series of tubes containing 1 μg of substrate DNA (λ DNA/Hind III fragments or pBR322/Hinf I fragments). After a 20-hours incubation under the recommended assay conditions, the characteristic banding pattern of the DNA substrate is determined by agarose gel/ethidium bromide electrophoresis. A sharp, unaltered pattern under these conditions is an indication that the enzyme preparation is free of detectable levels of nonspecific DNases. The results of the assay are reported on the Technical Data Sheet provided with each enzyme.

Assay for Nonspecific Endonucleases
The assay is performed in 50 μl of the reaction mixture, containing increasing amounts of the enzyme, its assay buffer and 1 μg of a supercoiled plasmid substrate. After a 20-hours incubation under appropriate conditions the DNA is examined for structural alterations in 1% agarose gel. The endodeoxyribonuclease activity is expressed as a percent of supercoiled DNA converted to open circular or linear form. The results of the assay are reported on the Technical Data Sheet provided with each enzyme.

Stability
The enzymes are stored at -20°C. The expiry date of the enzymes is indicated in the product Technical Data Sheet and on the vial label.
BIORON guarantees the maintenance of quality of DNA/RNA modifying enzymes until their respective expiry dates.

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