SE Buffers and Activity

All enzymes are supplied with an appropriate reaction buffer. For the majority of restriction enzymes we recommend to use 5 main buffers (B,G,O,W,Y), special buffers are used for EcoR I and Ksp22 I. The composition of the buffers can be found in the table below.

BSA (MBG): 10 mg/ml, Bovine Serum Albumine

Catalog #	Pack size	Price, Euro	SHOP
290001	1,0 ml	14,00	Add to cart
290005	5x 1,0 ml	50,00	Add to cart

Download: Datasheet (PDF)

Buffer B: 10 mM TrisHCI, pH 7,6, 10 mM MgCl₂, 1 mM DTT

Buffer G: 10 mM TrisHCI, pH 7,6, 10 mM MgCl₂, 1 mM DTT, 50 mM NaCl

Buffer O: 10 mM TrisHCI, pH 7,6, 10 mM MgCl₂, 1 mM DTT, 100 mM NaCl

Buffer W: 10 mM TrisHCI, pH 8,5, 10 mM MgCl₂, 1 mM DTT, 100 mM NaCl

Buffer Y: 33 mM TrisAcetate, pH 7,9, 10 mM MgCl₂, 1 mM DTT, 100 mM NaCl

Buffer 2 W: 20 mM TrisHCI, pH 8,5, 10 mM MgCl₂, 1 mM DTT, 200 mM NaCl

Buffer Eco RI: 100 mM TrisHCI, pH 7,6, 10 mM MgCl₂, 1 mM DTT, 50 mM NaCl

Buffer Ksp22 I: 10 mM TrisHCI, pH 7,6, 10 mM MgCl₂, 1 mM DTT, 200 mM KCl

Buffer N. Bst9: 10 mM TrisHCI, pH 8,5, 10 mM MgCl₂, 1 mM DTT, 150 mM KCl, 0,1mg/ml BSA

				Activity (% from maximum)
Enzyme	Recognition sequence	SE Buffer	BSA	B	G	O	W	Y	t, ⁰	Inacti- vation, 20 min
Acc113 I	AGT^ACT	Y	+	50-75	25-50	0-10	0-10	100	37	65°C
Acc16 I	TGC^GCA	W	-	50-75	75-100	25-50	100	75-100	37	65°C
Acc36 I	ACCTGC	Y	-	25-50	25-50	50-75	50-75	100	37	65°C
Acc65 I	G^GTACC	W	-	10-25	25-50	75-100	100	10-25	37	65°C
AccBS I	GAG^CGG	Y	-	75-100		25-50	25-50	100	37	65°C
Afe I	AGC^GCT	Y	-	10-25	25-50	75-100	75-100	100	37	65°C
Ahl I	A^CTAGT	B	+	100	75-100	25-50	25-50	75-100	37	80°C
Alu I	AG^CT	Y	-	75-100	75-100	10-25	50-75	100	37	65°C
Apa I	GGGCC^C	Y	+	50-75	25-50	0-10	0-10	100	37	65°C
AspS9 I	G^GNCC	W	-	50-75	50-75	75-100	100	50-75	37	65°C
AsuNH I	G^CTAGC	Y	+	75-100	50-75	0-10	0-10	100	37	65°C
BamH I	G^GATCC	G	+	25-50	100	75-100	75-100	25-50	37	65°C
Bgl I	GCCNNNN^NGGC	2W	-	50-75	50-75	0-10	75-100	25-50	37	65°C
Bgl II	A^GATCT	O	-	0-10	10-25	100	25-50	10-25	37	80°C
Bme18 I	G^GWCC	O	-	10-25	25-50	100	75-100	10-25	37	65°C
Bpu14 I	TT^CGAA	G	-	50-75	100	25-50	25-50	75-100	37	65°C
Bsa29 I	AT^CGAT	G	+	25-50	100	50-75	50-75	75-100	37	65°C
Bsc4 I	CCNNNNN^NNGG	W	+	75-100	75-100	50-75	100	25-50	55	80°C
Bse118 I	R^CCGGY	O	-	0-10	50-75	100	75-100	25-50	65	80°C
Bse21 I	CC^TNAGG	Y	-	50-75	50-75	10-25	25-50	100	37	80°C
Bse8 I	GATNN^NNATC	G	-	25-50	100	75-100	75-100	50-75	60	80°C
Bsp19 I	C^CATGG	2W	+	0-10	10-25	50-75	75-100	10-25	37	65°C
BssNA I	GTA^TAC	W	+	50-75	50-75	75-100	100	75-100	37	80°C
BssT1 I	C^CWWGG	2K	-	10-25	25-50	25-50	75-100	10-25	60	80°C
Bst4C I	ACN^GT	Y	-	75-100	75-100	10-25	25-50	100	65	80°C
BstDS I	C^CRYGG	Y	-	0-10	75-100	50-75	25-50	100	65	80°C
BstFN I	CG^CG	Y	-	75-100	50-75	25-50	25-50	100	60	80°C
BstH2 I	RGCGC^Y	Y	+	50-75	50-75	0-10	10-25	100	65	80°C
BstHH I	GCG^C	Y	+	75-100	50-75	25-50	50-75	100	50	65°C
CciN I	GC^GGCCGC	Y	-	25-50	50-75	75-100	75-100	100	37	65°C
Dra I	TTT^AAA	G	+	75-100	100	25-50	75-100	75-100	37	65°C
EcoR I	G^AATTC	*	+	50-75	75-100	75-100	100	50-75	37	65°C
EcoR V	GAT^ATC	W	+	0-10	25-50	50-74	100	25-50	37	80°C
FauND I	CA^TATG	Y	+	50-75	75-100	0-10	50-75	100	37	65°C
Hind III	A^AGCTT	W	+	10-25	25-50	0-10	100	0-10	37	80°C
Hinf I	G^ANTC	O	-	25-50	75-100	100	75-100	75-100	37	80°C
Hpa II	C^CGG	B	-	100	50-75	10-25	25-50	50-75	37	80°C
HspA I	G^CGC	Y	-	50-75	50-75	25-50	25-50	100	37	80°C
Kpn I	GGTAC^C	B	+	100	25-50	25-50	25-50	75-100	37	80°C
Ksp22 I	T^GATCA	G	+	50-75	100	50-75	50-75	25-50	37	65°C
Kzo9 I	^GATC	G	+	50-75	100	50-75	50-75	50-75	37	65°C
Mlu I	A^CGCGT	O	-	0-10	10-25	100	25-50	10-25	37	65°C
MroN I	G^CCGGC	B	-	100	50-75	10-25	0-10	10-25	37	80°C
Msp I	C^CGG	B	-	100	75-100	50-75	75-100	75-100	37	65°C
Nru I	TCG^CGA	W	-	0-10	10-25	75-100	100	10-25	37	80°C
Pce I	AGG^CCT	Y	-	75-100	75-100	50-75	25-50	100	50	80°C
Pci I	A^CATGT	O	-	50-75	75-100	100	75-100	50-75	37	65°C
Ple19 I	CGAT^CG	Y	-	75-100	75-100	25-50	25-50	100	37	65°C
Psp124B I	GAGCT^C	G	-	75-100	100	10-25	0-10	75-100	37	80°C
PspE I	G^GTNACC	B	-	100	50-75	25-50	50-75	50-75	37	65°C
PspOM I	G^GGCCC	Y	-	75-100	10-25	0-10	0-10	100	37	65°C
Pst I	CTGCA^G	O	+	10-25	25-50	100	25-50	25-50	37	80°C
Pvu II	CAG^CTG	G	+	25-50	100	25-50	25-50	25-50	37	80°C
Rsa I	GT^AC	B	-	100	50-75	0-10	50-75	75-100	37	80°C
Sal I	G^TCGAC	O	-	0-10	10-25	100	25-50	0-10	37	65°C
Sbf I	CCTGCA^GG	Y	-	75-100	50-75	0-10	0-10	100	37	80°C
Sfi I	GGCCNNNN^NGGCC	G	+	75-100	100	25-50	25-50	25-50	50	65°C
Sfr274 I	C^TCGAG	B	-	100	75-100	50-75	50-75	75-100	50	65°C
Sfr303 I	CCGC^GG	B	-	100	50-75	10-25	10-25	75-100	37	65°C
Sma I	CCC^GGG	Y	-	0-10	0-10	0-10	0-10	100	25	65°C
Smi I	ATTT^AAAT	O	+	25-50	25-50	100	75-100	25-50	37	65°C
Sph I	GCATG^C	G	+	25-50	100	75-100	75-100	50-75	37	65°C
Sse9 I	^AATT	B	+	100	75-100	50-75	50-75	75-100	55	65°C
Ssp I	AAT^ATT	K	-	75-100	50-75	25-50	50-75	75-100	37	65°C
Taq I	T^CGA	Y	+	50-75	75-100	75-100	50-75	100	65	80°C
Tru9 I	T^TAA	W	-	75-100	25-50	25-50	100	50-75	65	80°C
Vha464 I	C^TTAAG	G	-	50-75	100	25-50	10-25	75-100	37	65°C
Vne I	G^TGCAC	O	-	10-25	25-50	100	25-50	25-50	37	65°C
Vsp I	AT^TAAT	W		0-10	10-25	50-75	100	25-50	37	65°C
Xba I	T^CTAGA	O	-	75-100	75-100	100	50-75	75-100	37	65°C
NxBst9	GAGTC(4/-)	*	-	10-25	75-100	100	100	50-75	55	80°C

Hotstart Taq

Nucleotides

Cot-1 Human DNA

DFS Taq (16-S)

SE Buffers and Activity

Copyright © BIORON GmbH