endecnplse

DryMix SuperHot Taq

Catalog #Pack sizePrice, EuroSHOP
116101100 tubes (100 rcs)160,00Add to cart

For research use only

Opens internal link in current windowDatasheet: DryMix SuperHot Taq

THE TUBES MUST BE STORED AND SHIPPED AT ROOM TEMPERATURE

The qPCR GenePack Tubes kit contains

1.   100 MasterMix tubes (0,2 ml PCR tubes with optical caps for ABI- and BioRad-Cycler models).  Each Master Mix tube contains lyophilized mixture of "Hot-Start" Taq DNA polymerase for "QPCR" heat-sensitive Taq DNA polymerase inhibitors, dNTPs, MgCl2 all in amounts adjusted for optimal "Hot-Start" PCR in 25 ml volume.

2.   PCR diluent

Protocol for PCR with DryMix SuperHot Taq DNA Pol

  • Take the required amount of Master Mix tubes, label them according to the plan of your experiment. Always use 1 tube as positive control (template DNA) and one tube as negative control (no template DNA) in all your experiments
  • Centrifuge tubes briefly to place the lyophilized mixture on the bottoms of the tubes
  • Add 10 µl of PCR diluent in each tube
  • Add 5 µl of primer mix in each tube (recommended concentration - 0.1-0.5 mM of each)
  • Add 5 µl of sample (template DNA) in each tube
  • Centrifuge tubes briefly to get rid of possible air bubbles in the mixture
  • Run the PCR in thermocycler as usual.
to topprint