NEW!!!! Cytoplasmic and Nuclear Protein Extaction Kit
| Catalog # | Preparation | Price, Euro | SHOP |
| 810040 | 50 Preps | 99.00 | Add to cart |
Description:
This Kit is designed for rapid stepwise isolation of native, non-dentured cytoplasmic proteins and intact nuclei from cultured cells or tissue samples. The procedure, which is based on lysis with mild detergents, is fast and simple. The isolated proteins are ready for direct use in many proteomic or enzymatic activity applications, including immunoprecipitation, affinity purification and reporter gene assays. The isolated proteins are ready for direct use in many proteomic or enzymatic activity applications, including immunoprecipitation, affinity purification and reporter gene assays. Cell extacts prepared using this kit are compatible with common downstream applications, including 1D and 2D electrophoresis, Western blotting and electrophoretic mobility shift assays (EMSA). Isolated proteins are also compartible with quantification assays such as Bradford and Lowry.
The extracted nuclei can either be stored frozen in the provided Nuclei Storage Buffer or lysed with Nuclei Lysis Reagent. the intact nuclei further may be used for isolation of chromatin, histones and nuclear RNA. Nuclear extracts are used in DNA-protein interaction assays (EMSA), 2 D fractionation or other applications. The kit contains sufficient reagents for extraction of cytoplasmic and nuclear fractions from 50 samples of 50µl wet cell pellet or 100 ng of tissue.
Application:
Preparation of native, non-denatured nuclear and cytoplasmic protein extracts from mammalian cultured cells or tissue samples.
Components of the Kit:
- Cell Lysis Buffer
- Nuclei Washing Buffer
- Nuclei Storage Buffer
- Nuclei Lysis Reagent
- 1 M DTT
- Detailed Protokoll
Storage:
All components of the kit, except 1 M DTT, can be stored at 4°C for at least one year. Tore 1 M DTT in aliquots at -20°C.
