Bio Glutathione A for purifictation of GST-tagged Proteins
| Catalog # | Volume | Price, Euro | SHOP |
| 810010 | 10 ml | 180.00 | Add to cart |
| 810050 | 100 ml | 1600.00 | Add to cart |
Bio Glutathione A is an affinity chromatography medium which enables fast single step purification of glutathione-S-transferase (GST) fusion proteins and other glutathione-binding proteins. Purification of fusion proteins from a whole celllysate is based on the strong affinity of the GST moiety for glutathione, which is immobilized on agarose beads. GST-tagged proteins are eluted under mild, non-denaturing, conditions using neutral-pH buffers containing free glutathione. The purification proecess preserves protein antigenicity and functionality. If removal if the GST-tag is necessary, fusion preoteins may be cleaved using a site-specific protease.
Bio Glutathione A can be used in batch binding studies and/or packed bed chromatography system such as FPLC system.
Specification:
| Application | Batch binding Gravity flow column chomatography MPLC/FPLC |
| Form | 75% (v/v) aques suspension containing 20% ethanol 1ml of settled agarose beads (1 ml bed volume) corresponds to 1.333 ml of original 75% suspension |
| Matrix | 4% beaded agarose |
| Ligand | Glutathione, linked via sulfur atom |
| Spacer arm | 12 atoms |
| Bead size | 90 µm |
| Binding capacity | < 8mg recombinant GST/ml settled agarose |
| Recommended flow rates | 1 ml bed volume (column with 6.6 mm inner diameter): Sample loading 0.2-1.0 ml/min Washing and elution 1.0 ml/min 10 ml bed volume (column with 16 mm inner diameter): Sample loading 0.5-5.0 ml/min Washing and elution 5.0 ml/min |
| Maximum linear flow rate | 250 cm/h |
| Chemical stability | Bio Glutathione A withstands incubation in 0.1 M NaOH, 70% ethanol or 6 Mguanidine hydrochloride for 2 hours at room temperature without significatn losss of protein yield. |
| Storage temperature | 4-8 °C |
| Note | Do not autoclave the gel |
