endecnplse

Tth DNA Polymerase

Catalog #Pack sizePrice, EuroSHOP
104005500 u80,00Add to cart
1040252500 u320,00Add to cart

 

Opens internal link in current windowDatasheet: Tth DNA Polymerase
user manual: Tth DNA Polymerase

Opens internal link in current windowDeutsches Datenblatt: Tth DNA Polymerase

Description:
Tth DNA Polymerase is a thermostable enzyme of approximately 94 kDa isolated from eubacterium Thermus thermophilus strain HB8. This enzyme replicates DNA at 74°C (please see protocol below). It reveals RNA-dependent DNA-polymerase activity in the presence of Mn2+ ions. The concentration of RNA template for effective reverse transcription with Tth DNA polymerase should be higher if to compare with reverse transcription directed by Reverse Transcriptases (M-MuLV, AMV).

Concentration:
5000units/ml

Storage buffer:
10 TrisHCl, pH 7.5, 300 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol.

Unit definition:
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 70oC.

Supplied Reaction buffers:  

  1. RT-PCR buffer, one-step reaction (5 x): 250 mM bicine/KOH, pH 8.2 (25°C); 575 mM K-acetate, 40% glycerol (v/v)  
  2. 10 x Reverse transcription buffer: 100 mM Tris-HCl (pH 8.9 at 25°C), 900 mM KCl  
  3. 10 x Tth PCR buffer: 100 mM Tris-HCl (pH 8.9 at 25°C), 1M KCl, 500 µg/ml BSA, 0.5% Tween 20  and 15 mM MgCl2  
  4. Mn2+: 1 - 2 mM for RNA - dependent c-DNA synthesis. (supplied as MnCl2 in a separate tube).
  5. Mg2+: 3-6 mM for PCR reaction (supplied as MgCl2 in a separate tube).

Storage conditions:
-20oC  

to topprint