Psp DNA Polymerase
| Catalog # | Pack size | Price, Euro | SHOP |
| 108005 | 500 u | 170,00 | Add to cart |
| 108025 | 2500 u | 680,00 | Add to cart |
Datasheet: Psp DNA Polymerase
Description:
Psp DNA Polymerase Psp DNA Polymerase is a thermostable enzyme isolated from Pyrococcus species. The enzyme is quite similar to Pfu DNA Polymerase, it replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´-->3´ direction. Psp DNA Polymerase possesses 3'--> 5' exonuclease (proofreading) activity. Nucleotide misinsertions that may occur during polymerization are excised by the proofreading activity of the enzyme. Psp DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Psp DNA polymerase-generated PCR fragments are blunt-ended.
Supplied Buffers:
Incomplete (10x): 200 mM Tris-HCl (pH 8,8), 100 mM KCl, 100 mM (NH4)2SO4, 1,0 % Triton X-100, 1 mg/ml nulease-free BSA ; supplied with 1 ml of 25mM MgSO4 separately packed
Complete (10x): 200 mM Tris-HCl (pH 8,8), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1,0 % Triton X-100, 1 mg/ml nulease-free BSA
One tube with MgCl2 (100 mM)
One tube MgSO4 (25 mM)
