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Catalog 2007

SuperHOTTaq (Hot Start) DNA Polymerase

Applications:

- Complex genomic or cDNA templates 
- low copy numbers targets
- large numbers of thermal cycles
- multiplex PCR

Datasheet: Super HoTTaq (Hot Start) DNA polymerase
Deutsches Datenblatt: SuperHoTTaq (Hot Start) DNA Polymerase
Comparison various suppliers of Hot Start Taq DNA polymerase

Protocol for using SuperhoTTaq

Definition: SuperHoTTaq (Hot Start) DNA Polymerase is the optimized mixture of Taq DNA Polymerase and Anti-Taq DNA polymerase monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22^oC). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70oC. The PCR products obtained with SuperHOT Taq are free from unspecific products and from primer-dimers.  

Unit Definition:
One unit is defined as the amount of the enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72^oC.

Recommended Reaction Buffer (x1): 16 mM (NH₄)₂SO₄, 67 mM Tris-HCl (pH 8.8); 1,5-7 mM MgCl₂ 0.01% Tween-20

Together with the Enzyme we deliver for free:

- Reaction buffer (x10) "incomplete" supplied: 160 mM (NH₄)₂SO₄, 670 mM TrisHCl pH 8,8, 0,1%
  Tween-20, plus one Tube MgCl₂ (100 mM)
- Reaction buffer (x10) "complete" supplied: 160 mM (NH₄)₂SO₄, 670 mM TrisHCl pH 8,8, 0,1%
  Tween-20, 25 mM MgCl₂ 
- Reaction buffer (10x) "complete II KCl" supplied: 500 mM KCl, 100 mM Tris-HCl pH 8,8, 0,1%   
  Tween-20, 15 mM MgCl₂
- MgCL₂ (100mM)

Storage Buffer: 10 mM Tris-HCl (pH 7.0); 50 mM KCl; 0.1 mM EDTA; 50% glycerol
Storage Conditions: -20^oC

Concentration: 5000 units/ml

Recommended PCR conditions:
Use PCR conditions optimized for Taq DNA polymerase. In the case of low amount of DNA template, additional cycles may be used.

Recently SuperHotTaq DNA Polymerase and 15 others Thermophilic DNA polymerases from the major suppliers of enzymes for molecular biology  were tested for performance and sensitivity in Mycoplasma pneumonia and Mycoplasma genitalium detection tests. The sample contains dilutions of tested DNA and constant amount of positive control D. SuperHot Taq DNA Polymerase from Bioron was shown to be the best in comparison with all competitor's enzymes.

1. 100bp DNA ladder
2. Negative control
3. M. genitalium DNA, no dilution
4. M. genitalium DNA 10^-1 dilution
5. M.genitalium DNA 10^-2 dilution
6. M. genitalium DNA 10^-3 dilution
7. M. genitalium DNA 10^-4 dilution

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