SuperHOTTaq (Hot Start) DNA Polymerase
| Catalog # | Pack size | Konz | Price, Euro | SHOP |
| 119002 | 200 u | 5 u/µl | 62,00 | Add to cart |
| 119010 | 1000 u | 5 u/µl | 248,00 | Add to cart |
| 119010L (high conc.) | 2000 u | 14-18 u/µl | 744,00 | Add to cart |
Datasheet: SuperHOTTaq DNA Polymerase
Applications
- Hotstart PCR
- Realtime PCR
- Amplification of complex genomic and cDNA templates
- Multiplex PCR
- high specifity PCR
Description: SuperHoTTaq (Hot Start) DNA Polymerase is the optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22°C). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70°C. The PCR 
products obtained with SuperHOT Taq are free from unspecific products and from primer-dimers.
Features
- reliable and reproducible quantification in qPCR
- perfect for Realtime PCR
- especially for diagnostic purposes
- reaction set-up at room temperature
- activation of enzyme during first heating
- no change or optimization of protocol necessary
- high specifity, reduced primer mismatch or dimers
Concentration: 5 units/µl (or high concentrated 17,5 u/µl)
Storage Conditions: -20oC
Comparison Test
Recently SuperHotTaq DNA Polymerase and 15 others Thermophilic DNA polymerases from the major suppliers of enzymes for molecular biology were tested for performance and sensitivity in Mycoplasma pneumonia and Mycoplasma genitalium detection tests. The sample contains dilutions of tested DNA and constant amount of positive control D. SuperHot Taq DNA Polymerase from Bioron was shown to be the best in comparison with all competitor's enzymes. 
from left to right:
1. 100bp DNA ladder
2. Negative control
3. M. genitalium DNA, no dilution
4. M. genitalium DNA 10-1 dilution
5. M.genitalium DNA 10-2 dilution
6. M. genitalium DNA 10-3 dilution
7. M. genitalium DNA 10-4 dilution
