PCR Cloning Kit (blunt-end)
| Catalog # | Price, Euro | SHOP |
| 114001 | EUR 79,00 20 reactions | Add to cart |
Datasheet: PCR "blunt-end" Cloning kit
Vector-Sequence sheet PCR "blunt-end" cloning Kit
Cloning map: PCR "blunt-end" cloning Kit
Cloning vector sequence: PCR "blunt-end" Cloning Kit
End-polishing or blunt-end cloning kit contains all components for the 3'-end-polishing of amplicons (if the amplicons contain 3'-overhang) and blunt-end cloning of the resultant fragments.
The kit is useful for blunt-end fragment preparation from fragments with 5' or 3'-overhangs of all types (obtained after restriction endonucleases digestion, after exonucleases treatment, after unspecific degradation etc.) with the possibility of blunt-end cloning of the end-polished fragments.
The plasmid used for cloning is Ampr, the recombinant plasmid may be selected by Lac+/Lac- phenotype detection (blue/white selection on the X-gal/IPTG - containing plates).
The kit contains (for 20 reactions) |
10 x Psp buffer |
