Ron’s Total RNA Kit
| Catalog no. | Pack size | Price, Euro | Shop |
| 806834 | 50 preps | 169,00 | Add to cart |
| 806834L | 5x50 preps | 811,00 | Add to card |
Manual Total RNA isolation Kit
Introduction
The Ron’s Total RNA Kits provide a rapid and easy method for the isolation of up to 100 µg of total RNA from eukaryotic cells and tissues. These kits allow processing of a single or multiple samples in less than 30 min.
Normally, up to 1 x 107 cells or 40 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol. RNA purified using the Ron’s Total RNA Kits is ready for applications such as RT-PCR, Northern blotting, poly(A)+-RNA (mRNA) purification, nuclease protection assays and in vitro translation.
Principle
The Ron’s Total RNA Kits use the reversible binding properties of the HiBind matrix, a new silica-based material. This is combined with the speed of mini-column spin technology. A specifically formulated high salt buffer system allows more than 100 µg of RNA molecules larger than 200 bases to bind to the matrix. Cells or tissues are first lysed under denaturing conditions that practically inactivate RNases. Samples are then applied to the HiBind spin columns to which total RNA binds, while cellular debris and other contaminants are effectively washed out. High quality RNA is finally eluted in RNase-free sterile water.
