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FauND I

Catalog #Pack sizePrice, EuroSHOP
278005500 u16,00Add to cart
2780252500 u64,00Add to cart

Opens internal link in current windowDownload: Datasheet FauND I (Nde I)


CA↓TATG

GTAT­↑AC

Purified from Flavobacterium aquatili ND

Isoschizomer Nde I

Concentration:
10-20000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37oC

Recommended buffer:
B

Ligation/recutting assay:
After 3-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 6 units of enzyme at 37oC.

Heat inactivation (20 min, 65oC):
Yes

Note! The enzyme is very sensitive to DNA impurities.

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